KitContents
| 96wellPermethylationplate | 1Plate |
| Dimethylsulfoxide(DMSO) | 40.0ml |
| Dichloromethane(DCM) | 60.0ml |
| 96wellBalancePlate | 1Plate |
| 96wellPlateLid | 1Lid/Mat |
Thepermethylationplate(LC-PERMET-96)isscalableandcanbeusedforanynumberofsamplesbetween1and96.Storetheunusedkitreagentsandtheunusedplatewellsintherecommendedstorageconditionstoenabletheiruseforfurthersamplepreparationatalaterdate.
Note:Duetoshippingrestrictionsofmethyliodide/iodomethane,weareunabletoprovidethiscomponentwiththekit.Werecommendpurchasingiodomethanewithpurity≥99.0%(GC)fromyourlocalchemicalprovider.IodomethaneSynonym:methyliodideCASNumber:74-88-4
| a.Preparationofglycans | Asrequired |
| b.AdditionofDMSOandsampletoplate | 15minutes |
| c.Incubation | 15minutes |
| d.AdditionofMeItopermethylationplate | 15minutes |
| e.Incubation | 60minutes |
| a.Extractionofsamples | 3-4hours |
| b.Dryingpermethylatedsamples | 10-30minutes |
| c.Additionof70%MeOHforstorage | 10-20minutes |
PermethylationReferences
A.Dell;A.J.Reaseon;Kay-HooiKhoo;M.Panico;A.McDowellandH.R.MorrisMassSpectrometryofCarbohydratecontainingBiopolymerMethodsinEnzymology230:108-132(1994)
I.Ciucanu;C.E.CostelloEliminationofOxidativeDegradationduringtheper-O-MethylationofCarbohydratesJ.Am.Chem.Soc.125:16213-16219(2003)
I.CiucanuPer-O-MethylationreactionofstructuralanalysisofcarbohydratesbymassspectrometryAnalyticaChimicaActa576:147-155(2006)
I.Ciucanu;R.CapritaPer-O-MethylationofneutralcarbohydratesdirectlyfromaqueoussamplesforgaschromatographyandmassspectrometryanalysisAnalyticaChimicaActa585:81-85(2007)
Shubhakar,A.,Kozak,R.P.,Reiding,K.R.,Royle,L.,Spencer,D.I.R.,Fernandes,D.L.,Wuhrer,M.AutomatedHigh-ThroughputPermethylationforGlycosylationAnalysisofBIOLOGicsusingMALDI-TOF-MSAnalyticalChemistry.acs.analchem.6b01639(2016).
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